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產(chǎn)品貨號(hào)Reference:               CAN-PRE-4500
檢測(cè)方法Kit Type:                  Competitive ELISA
規(guī)格Kit Size:                          96-well break-apart microplate
靈敏度Sensitivity:                 0.05 ng/mL
檢測(cè)樣本Sample Type:         Human serum / 50 μL
檢測(cè)范圍Calibrator Range:  0.1–25.6 ng/mL
檢測(cè)時(shí)間Total Assay Time:  75 minutes

背景介紹：

孕烯醇酮(3β-Hydroxypregn-5-en-20-one)是甾體合成途徑中第一個(gè)由膽固醇衍生而來(lái)的甾體，是所有腎上腺和性腺類固醇的共同前體。它的產(chǎn)生是通過(guò)P-450SCC酶切割膽固醇C-20側(cè)鏈而在線粒體中產(chǎn)生的。孕烯醇酮一旦產(chǎn)生，可通過(guò)兩條甾體合成途徑加以利用。孕烯醇酮可以通過(guò)17-α-羥化酶的酶促作用轉(zhuǎn)化為17-OH孕烯醇酮，也可以通過(guò)3-β-羥基類固醇脫氫酶的酶促作用轉(zhuǎn)化為孕酮。
Pregnenolone (3β-hydroxypregn-5-en-20-one) is the first steroid to be derived from cholesterol in the pathway of steroidogenesis, and it is the common precursor for all of the adrenal and gonadal steroids. Its production occurs in the mitochondrion by cleavage of the C-20 side chain of cholesterol by the P-450SCC enzyme. Once produced, pregnenolone may be utilized by two pathways of steroidogenesis. Pregnenolone may either be converted to 17-OH pregnenolone via the enzymatic action of 17α-hydroxylase or to progesterone via the enzymatic action of 3β-hydroxysteroid dehydrogenase.