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產(chǎn)品貨號(hào)Reference:              CAN-RT3-100
檢測(cè)方法Kit Type:                 Competitive ELISA
規(guī)格Kit Size:                         96-well break-apart microplate
靈敏度Sensitivity:                 0.009 ng/mL
檢測(cè)樣本Sample Type:          Human serum or plasma / 25 μL
檢測(cè)范圍Calibrator Range:   0.02–2 ng/mL
檢測(cè)時(shí)間Total Assay Time:  105 minutes

背景介紹：
DBC的RT3 ELISA試劑盒采用競(jìng)爭(zhēng)性酶聯(lián)免疫分析方法，其抗原(存在于校準(zhǔn)器、對(duì)照和病人標(biāo)本中)與生物素標(biāo)記的抗原(RT3-生物素結(jié)合物)競(jìng)爭(zhēng)，以獲得包被在微孔板上的有限數(shù)量的抗體。孵育1小時(shí)后，第一次洗滌，去除未粘結(jié)的材料，加入鏈霉親和素-HRP結(jié)合物，孵育30分鐘。在第二次洗滌之后，添加TMB基板。酶反應(yīng)通過加入停止溶液而終止，在停止溶液的基礎(chǔ)上用微孔板讀取器測(cè)量顏色強(qiáng)度。顯色強(qiáng)度與樣品中rT3的濃度成反比。與樣品同時(shí)運(yùn)行的一套試劑盒校準(zhǔn)器用于繪制校準(zhǔn)曲線，并測(cè)定樣品和對(duì)照品中rT3的濃度。
The DBC rT3 ELISA is a competitive enzyme immunoassay, where the antigen (rT3 present in calibrators, controls and patient samples) competes with a biotin-labelled antigen (rT3-Biotin conjugate) for a limited quantity of antibody which is coated on the microplate wells. After one hour incubation followed by the first washing, unbound materials are removed and a Streptavidin-HRP conjugate is added and incubated for 30 minutes. Following a second washing, the TMB substrate is added. The enzymatic reaction is terminated by addition of the stopping solution, upon which the colour intensity is measured with a microplate reader. The colour intensity is inversely proportional to the concentration of rT3 in the sample. The set of kit calibrators that are run simultaneously with the samples is used to plot a calibration curve and determine the concentration of rT3 in samples and controls.

注：本產(chǎn)品僅用于科研用途，不能直接用于人體或臨床診斷使用。